A general purpose RNA-cleaving DNA enzyme

A general purpose RNA-cleaving DNA enzyme (antisenseycatalytic DNAyin vitro selectionyRNA cleavage)

An in vitro selection procedure was used to develop a DNA enzyme that can be made to cleave almost any targeted RNA substrate under simulated physiological conditions. The enzyme is comprised of a catalytic domain of 15 deoxynucleotides, f lanked by two substrate-recognition domains of seven to eight deoxynucleotides each. The RNA substrate is bound through Watson–Crick base pairing and is clea...

SECONDARY STRUCTURE CHARACTERIZATION OF pH6DZ1, A FLUORESCENCE SIGNALING AND RNA CLEAVING DNA ENZYME SECONDARY STRUCTURE CHARACTERIZATION OF pH6DZ1, A FLUORESCENCE SIGNALING AND RNA CLEAVING DNA ENZYME

pH6DZ1 is a synthetic deoxyribozyme that is able to couple catalysis with fluorescence signal generation. This deoxyribozyme has the ability to cleave itself at a lone ribonucleotide that is present between a pair of deoxyribothymidines, one modified with a fluorophore (fluorescein) and the other with a quencher (DABCYL). Herein we report on the sequence truncation and secondary structure chara...

Cleaving DNA with DNA.

A DNA structure is described that can cleave single-stranded DNA oligonucleotides in the presence of ionic copper. This "deoxyribozyme" can self-cleave or can operate as a bimolecular complex that simultaneously makes use of duplex and triplex interactions to bind and cleave separate DNA substrates. Bimolecular deoxyribozyme-mediated strand scission proceeds with a kobs of 0.2 min-1, whereas th...

Acetylacetone-cleaving enzyme Dke1: a novel C-C-bond-cleaving enzyme from Acinetobacter johnsonii.

The toxicity of acetylacetone has been demonstrated in various studies. Little is known, however, about metabolic pathways for its detoxification or mineralization. Data presented here describe for the first time the microbial degradation of acetylacetone and the characterization of a novel enzyme that initiates the metabolic pathway. From an Acinetobacter johnsonii strain that grew with acetyl...

General-Purpose Computation without General-Purpose Processors